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KMID : 0545120080180121927
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 12 p.1927 ~ p.1931
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Optimization of the Process for Biodiesel Production Using a Mixture of Immobilized Rhizopus oryzae and Candida rugosa Lipases
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Lee Jong-Ho
Lee Dong-Hwan
Lim Jung-Soo
Um Byung-Hwan
Park Chul-Hwan
Kang Seong-Woo
Kim Seung-Wook
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Abstract
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In this study, the enzymatic process for biodiesel production was optimized using a mixture of immobilized Rhizopus oryzae and Candida rugosa lipases. The optimal temperature and agitation speed for biodiesel production were 45oC and 300 rpm, respectively. The optimal ratio of R. oryzae and C. rugosa lipases in the mixture was 3:1 (w:w). When 3mmol of methanol was the initial reaction medium and 3mmol of methanol was added every 1.5 h during biodiesel production, biodiesel conversion was over 98% at 4 h. In addition, when the immobilized lipase mixture was reused, biodiesel conversion exceeded 80% after 5 reuses.
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KEYWORD
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Biodiesel, immobilization, Rhizopus oryzae lipase, Candida rugosa lipase
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